Rapid affinity purification process of EPO form urine as pre-treatment step for the IEF EPO doping test

Project Title: Rapid affinity purification process of EPO from urine as pre-treatment step for the IEF EPO doping test

Researcher: J. Carlsson

Institution: Uppsala University, Sweden

Year accepted: 2007

Summary: The first study of the affinity purification of EPO from urine, using the novel anti-EPO monolith, showed high recoveries of EPO as well as maintained position of the isoform bands obtained in the IEF method. The content of urine proteins, besides EPO, was heavily reduced after the affinity purification step while EPO was concentrated 50 times. In this project further improvements will be introduced in the affinity purification process to obtain an easy-to-use regime optimised for the IEF method. In the next step a production project will be started in order to implement the procedure at some doping laboratories during 2008.