In force Publication date 24 Jul 18
Ultra-Sensitive Mass Spectrometric Detection of a rEPO Specific O-glycopeptide as an Unambiguous Proof of Doping—Follow-up (GOpep2)
Resource description
Project Title: Ultra-Sensitive Mass Spectrometric Detection of a rEPO Specific O-glycopeptide as an Unambiguous Proof of Doping—Follow-up (GOpep2)
Researcher: Dr. J. Pascual, Dr. J. Segura
Institution: Fundacio IMIM (FIMIM), Spain
Year accepted: 2017
Summary: The hypothesis of this project is that MS sensitivity has reached a status in which EPO glycopeptides, particularly O-glycopeptides as they present lower heterogeneity, are detectable in urine or blood samples. Using a proper combination of specific peptide immunopurification with other desalting techniques, matrix effects can be avoided and the required sensitivity for the EPO O-glycopeptide containing the non-human tag (Neu5Gc) reached.