In force Publication date 13 Jul 15

Ultra-Sensitive Mass Spectrometric Detection of an rEPO Specific O-glycopeptide as an Unambiguous Proof of Doping (acronym: GOpep)

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Project Title: Ultra-sensitive mass spectrometric detection of an rEPO specific O-glycopeptide as an unambiguous proof of doping (acronym: GOpep)

Researchers: Dr. J. Pascual, Dr R. Gutierrez-Gallego, DR. V. Sanz-Nebot

Institution: Fundació Institut Mar d’Investigacions Mèdiques IMIM, Spain

Year accepted: 2013

Summary: The objective of the project are:

  • To develop an optimized method of immunopurification and enzymatic digestion of rEPOs.
  • To optimize separation and MS conditions for each O-glycopeptide and scale-up sensitivity using the latest state-of-the-art MS instrumentation from AB Sciex.

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