Structural elucidation of the glycans present in human urinary EPO

Project Title: Structural elucidation of the glycans present in human urinary EPO

Researchers: J. A. Pascual, E. L. Escorihuela, J. Segura, R. Gutierrez Gallego

Institution: Fundacio IMIM, Barcelona, Spain

Year accepted: 2006

Summary:  The aims of the present project are:

• To develop a purification procedure for endogenous urinary EPO.
• To perform structural elucidation of the EPO released glycans based on:
  • Mass spectrometric analysis (MALDI-TOF, ESI-Q-TOF)
  • Monosaccharide analysis (GC/MS)
  • Sialic acid speciation (reverse phase HPLC)
  • Weak anion exchange (WAX) profiles
  • Normal phase LC profiling
  • RAAM (reagent array analysis method)