In force Publication date 09 Feb 16
A Secretagogue Chemiluminescent Assay
Resource description
Project Title: A Secretagogue Chemiluminescent Assay
Researchers: Pr. R. Gutierrez-Gallego, Pr. J. Segura, Pr. P. Ferro Gallego
Institution: Fundació Institut Mar d’Investigacions Mèdiques (IMIM), Spain
Year accepted: 2013
Summary: The main objectives of this project were:
- Obtention of appropriate chemiluminescent tags bound to streptravidin and synthesis, purification, and characterisation of streptavidin conjugated to acridinium, not commercially available.
- Set-up of a competition assay protocol employing different chemiluminescent tags. - Comparison of the radiotracer and the chemiluminescent tracers.
- Assessment of the chemiluminescent competition assay in urine samples.