“Screening of testosterone misuse by metabolites resistant to enzymatic hydrolysis” and “Characterization, elucidation and synthesis of a potential marker for testosterone misuse”

Project Title: “Screening of testosterone misuse by metabolites resistant to enzymatic hydrolysis” and “Characterization, elucidation and synthesis of a potential marker for testosterone misuse”

Researchers: Dr. O.J. Pozo, Dr. J. Marcos

Institution: Fundacio IMIM, Spain

Year accepted: 2014

Summary: The project has two main goals. Firstly, the project aims to evaluate the potential of 3α-glucuronide-6β-hydroxyandrosterone (6OH-A-3G) and 3αglucuronide-6β-hydroxyetiocholanolone (6OH-Etio-3G) for the screening of endogenous anabolic androgenic steroid misuse. For this first purpose, a quantitative method validated during the project 12A13OP will be applied to several samples from excretion studies already available in our laboratory. Secondly, the project also aims to characterize, elucidate and synthesize the marker M170T298. For this purpose, a large range of analytical strategies will be applied e.g. different derivatization steps, an in-depth study of accurate mass and tandem mass spectra.