Qualitative and quantitative determination of Insulin like Growth Factor-I (IGF-I) or synthetic analogues in human plasma

Project Title: Qualitative and quantitative determination of Insulin-Like Growth Factor-I (IGF-I) or synthetic analogues in human plasma

Researchers: M. Thevis, W. Schänzer

Institution: German Sport University, Cologne, Germany

Year accepted: 2005

Summary: The amino acid composition of recombinant IGF-I is identical to that of endogenously produced IGF-I, and preliminary approaches to reveal IGF-I misuse are based on its quantification. By means of mass spectrometry, a precise determination of IGF-I amounts in human plasma is aspired in addition to an unambiguous identification of this peptide. Moreover, synthetic analogues such as R3 -IGF-I and long IGF-I are commercially available, and strategies enabling the detection of three structurally related compounds are required. A selective isolation of IHG-I and analogues from human plasma is possible by means of immunoaffinity extraction and subsequent liquid chromatography coupled to mass spectrometry provides substantial data allowing a qualitative and quantitative determination of target analyses.