In force Publication date 13 Apr 15
Identification and mass spectrometric characterization of urinary proteins other than EPO binding to clone AE7A5 anti-human EPO antibody
Project Title: Identification and mass spectrometric characterization of urinary proteins other than EPO binding to clone AE7A5 anti-human EPO antibody
Researchers: C. Reichel, G. Gmeiner, V. Jordan, M. Watzinger, R. Kulovics
Institution: Austrian Research Centers GmbH-ARC, Seibersdorf, Austria
Year accepted: 2007
Summary: The purpose of this project is a detailed study on the specificity of the clone AE7A5 antibody. According to the WADA technical document on the “Harmonization of the method for the identification of epoetin alfa and beta (EPO) and darbepoetin alfa (NESP) by IEF-double blotting and chemiluminescent detection” the IEF-method “relies on the particular specificity of the monoclonal antibody with which it was developed (clone AE7A5)” and “this antibody is considered a critical reagent and shall not be changed”. However, during SDS-PAGE experiments we were able to show that AE7A5 also binds to urinary proteins other than erythropoietin and Zinc-alpha-2-glycoprotein. These proteins might interfere with the interpretation of the EPO-IEF profiles.
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