Development of reliable blood tests for the detection of gene doping after intramuscular injection of recombinant adeno-associated viral vectors

Project Title: Development of reliable blood tests for the detection of gene doping after intramuscular injection of recombinant adeno-associated viral vectors

Researchers: Dr. Snyder (University of Florida, USA), Dr. Mouillier (University of Nantes, France), Dr. Simon (Johannes Gutenberg-University, Germany)

Year approved: 2011

Summary:

• Aim 1: Evaluate a combined assay format that is based on real-time PCR and ITC readout with a nested PCR pre-step to improve sensitivity.
• Aim 2: Assay analytical validation for specificity, sensitivity, and ruggedness (multiple labs, multiple operators, repeats and replicates) and preliminary pre-clinical performance validation.