Development of a prototype blood-based test for exogenous erythropoietin activity based on transcriptional profiling

Project Title: Development of a prototype blood-based test for endogenous erythropoietin activity based on transcriptional profiling

Researchers: J. Rupert, D. McKenzie

Institution: University of British Columbia, Vancouver, Canada

Year accepted: 2005

Summary: In our project, Serial Analysis Gene Expression is being used to compare the transcriptional profile of the hypoxia response to that of foreign EPO activity. We hypothesize that comparing these profiles will identify differentially expressed genes that can be used to develop a diagnostic test. We will identify these genes in a mouse model, locate and characterize their human counterparts, and incorporate the latter into blood-based test for exogenous EPO expression.