In force Publication date 13 Apr 15
Detection of autologous blood transfusion using activated red blood cells (the red blood cells eNOS system)
Resource description
Project Title: Detection of autologous blood transfusion using activated red blood cells (the red blood cells eNOS system)
Researchers: P. Platen (Ruhr University of Bochum, Germany), W. Bloch, W. Schänzer, S. Voss (German Sport University, Cologne, Germany)
Year accepted: 2008
Summary: We will investigate 24 healthy male and female physically active subjects prior to 500 ml blood donation, as well as prior to and several days after re-infusion of the autologous blood concentrate after storage of 2 to 4 weeks. RBC NOS activity and adhesion receptors will be analysed in venous blood samples by means of immunohistochemistry and FACS. Furthermore, changes in blood total haemoglobin mass (tHb) will be analysed several times from prior to the donation until 2 weeks after re-infusion by means of optimized CO rebreathing method, allowing a quantification of the percentage of the re-infused amount of blood. Aerobic performance (V’O2max) will be tested several times for the analysis of the physiological effects of the procedures.