The partitioning of prohibited substances between erythrocytes and blood plasma for the assessment of doping-relevant concentrations in dried blood spots

Code: 252A09MT

Many substances (also some of those belonging to the WADA Prohibited List) tend to enrich (or deplete) in red blood cells and, thus, will show significantly deviating concentrations in whole blood (such as DBS) and plasma (or serum) samples. For an accurate evaluation and interpretation of analytical results obtained from DBS analyses, the information about the RBC-to-plasma-ratio for each substance is crucial, not only in the context of quantitative result management. The RBC-to-plasma-ratio can be characterized by a simple and straight forward in-vitro approach with incubating the substances of interest in whole blood, followed by the separate analysis of the cells and the plasma fractions. With those experiments, the partitioning of nearly all prohibited substances between erythrocytes and blood plasma can be determined. These data will be of substantial importance for future result management processes where concentrations of MRL- and threshold-substances in DBS are to be evaluated.