Combined screening on diuretics and heavy volatile nitrogen containing doping substances, investigations on the metabolism and synthesis of phase II metabolites

Project Title: Combined screening on diuretics and heavy volatile nitrogen containing doping substances, investigations on the metabolism and synthesis of phase II-metabolites

Researchers: W. Schänzer, M. Parr

Institution: German Sport University, Cologne, Germany

Year accepted: 2005

Summary: The project aims at combining the screening methods for diuretics, beta-2-agonists, heavy volatile stimulants and narcotics in one LC-MS/MS method. To avoid problems with the stability of diuretics during the hydrolysis, direct analysis of the excreted metabolites (where applicable as intact conjugates) is mandatory. This is also beneficial because relatively clean extracts are obtained and the time consuming hydrolysis is eliminated.