En vigueur

Final Validation for Implementation of GH Detection First Step: Collection Scenarios

Investigateur principal
M. Saugy
Laboratoire Suisse d'Analyse du Dopage
Année approuvée
Autres facteurs de croissance

Description du projet

Code: T08A02MS

The aim of the project is to evaluate the robustness of the method with athlete’s samples regarding some collection scenarios and to compare the results obtained in different transport and storage conditions on serum samples. The final aim of the global project is to obtain information on sampling practices also called collection scenarios based on supporting data.

Main Findings: 

• For samples separated immediately after clotting, rhGH detection kit appears to have a slightly better sensitivity when samples are stored frozen compared to cooled.
• For samples arriving after 4 days (96h) and centrifuged upon reception, no differences exist for ratio if samples are analyzed immediately or after 7 days if frozen storage is applied.
• For samples separated immediately after clotting, storage must be made frozen. Under cool conditions, ratios is lower after ten days compared to one day. If frozen, ratios do not present any significant difference when analyzed after one day or ten days.
• If delay between end of collection and centrifugation is below 24 hours, no significant differences of ratio is observed between delays.
• Longer delays (up to 3 days; 72h) leads to lower ratio, but difference is close to analytical variation predicted by rhGH kits supplier’s experts.
• Later, ratios get significantly lower and tends to continuously decrease with time.