Improving the 19-Norsteroids abuse detection

Code: 23A10XT

Isotope ratio mass spectrometry's (IRMS) efficiency for detecting pseudo-endogenous steroids depends on the difference between the isotopic labeling of the formulations available in the market and the natural isotopic labeling of the endogenously produced steroids by the athletes. The more frequent presence of pharmaceutical formulations with values close to the endogenous ones is becoming challenging and in some populations makes the current detection approach useless. This phenomenon already observed for testosterone preparations lead WADA to establish some countermeasures as the ABP endocrine module (for their indirect detection) or the analysis of the actual testosterone esters (the most frequent way of administration) in blood samples or dried blood spots (DBS). We are here proposing a study directed to verify the confirmation capacity of nandrolone esters in DBS or blood and the effect of the administration over the parameters of the endocrine module. This will be compared with the current approach by GC-MS/MS (for the Initial testing procedure- ITP) and GC-C-IRMS (for the confirmation procedure- CP). Formulations not detectable with the current approach will be included in the study.