Development of detection method of hydrolysed rapid-acting insulin analogues in human urine by ion trap mass spectrometry

Project Title: Development of detection method of hydrolysed rapid-acting insulin analogues in human urine by ion trap mass spectrometry

Researcher: C. Ayotte

Institution: INRS-Institut Armand Frappier, Pointe Claire, Quebec, Canada

Year accepted: 2007

Summary: It is proposed that hydrolysis can be performed prior to analysis on an ion trap to monitor the smaller peptide containing the susceptible amino acid interchange, and then fragmentation pattern can qualify the analogues. The goal of this project is to develop a detection method for hydrolysis products of insulin and rapid-acting analogues using an ion trap mass spectrometer. Complementarily, a simpler purification method using single-use immunoprecipitation will be tentatively developed and compared with the 3-steps approach